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. 2012 Feb 27;103(4):670–676. doi: 10.1111/j.1349-7006.2012.02213.x

Figure 2.

Figure 2

Measurement of 5‐hmC in colorectal cancers (CRCs). (A) Dot blot analysis using anti‐5hmC antibody. (B) Genomic DNA from embryonic stem (ES) cells was subjected to dot blot. Loading control is shown by the methylene blue (MB) staining. Densitometry measurements against logarithmic DNA amount were plotted. (C) Quantitative assessment of 5‐hmC in ES cell DNA (160 ng). ES cells were incubated with or without leukemia inhibitory factor (LIF) for 5 days. (D) Detection of 5‐hmC in 22 pairs of clinical CRCs (T; right column on each membrane) and adjacent non‐tumorous tissue (N; left) using dot blot. Twofold diluted DNA was also spotted in the second row on the same membrane. Loading control is shown by MB staining of undiluted DNA samples. (E) Classification of CRCs according to the 5‐hmC level.