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. 2012 Sep 25;103(11):1929–1937. doi: 10.1111/j.1349-7006.2012.02409.x

Figure 2.

Figure 2

Effect of SB365 on apoptosis of Huh‐7 cells. (A) Huh‐7 cells were treated with SB365 (0, 0.1, 1, 5 and 10 μM) for 24 h, stained with propidium iodide (PI) and analyzed on a FACSCalibur flow cytometer. (B) The induction of apoptosis by SB365 was conducted by DAPI and TUNEL staining, which were photographed at ×100 and ×400 magnification. (C) The expression of Bcl‐2, Bax and cleaved caspase‐3 were determined by western blotting in cells treated with SB365 at the indicated doses for 24 h.