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Effect of SB365 on the PI3K/Akt/mTOR pathway signalling in Huh‐7 hepatocellular carcinoma cells. (A) Cells were treated with SB365 with various doses (0.1–10 μM). Western blotting experiment for p‐Akt, p‐mTOR and p‐p70S6K were performed. (B) Immunofluorescent imaging for target proteins of PI3K/Akt/mTOR pathway after SB365 treatment (10 μM). For labelling, anti‐rabbit antibodies against p‐Akt, p‐mTOR, and p‐70S6K were used (×400 magnification).
