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. 2012 Jul 24;103(10):1795–1802. doi: 10.1111/j.1349-7006.2012.02363.x

Figure 2.

Figure 2

Characterization of PC‐9/ER3 cells. (A) The T790M mutation was not found in PC‐9/ER3 cells by direct sequencing. (B) MET gene copy number was examined by quantitative PCR using genomic DNA extracted from PC‐9 and PC‐9/ER3 cells. MET gene copy number relative to GAPDH was measured in three independent experiments. Bars, SD. (C) Cells were incubated with or without 2 μM erlotinib for 6 h and subjected to western blotting. pEGFR was suppressed, and PTEN expression was similar in both cell lines.