Figure 1.
Disorganized epithelial morphology in the ρ0 cells. (a) NMuMG cells were treated with EtBr (250 ng/mL) for the indicated periods and observed by phase contrast microscopy. (b),(c) Treated cells were examined by indirect immunofluorescence labeling using specific antibodies. Actin fibers were stained with florescence‐labeled phalloidin and the nuclei with DAPI. N: Control. ρ0: 6‐day treatment with EtBr. (d) Cell lysate was examined by immunoblotting with the antibodies as indicated. GAPDH was used as the loading control. (e) Total RNA was extracted and analyzed by real‐time RT‐PCR, as described in the Materials and Methods section. The PCR products derived from each mRNA were normalized to that derived from GAPDH in the same sample and are shown as a ratio relative to the control. The difference in ITGA1 expression in control and ρ0 samples was assessed by t‐test. #P < 2 × 10−6, *P < 0.05.