Figure 4.

High mobility group AT‐hook 2 (HMGA2) upregulated downstream in the cAMP‐responsive element‐binding protein (CREB) pathway. (a–c) Cell lysates from control (N) and the ρ0 cells were subjected to immunoblotting using an antibody specific for HMGA2, as above. In (a), shRNA for CREB or the control was expressed under Dox− conditions. Cells were treated with TGF‐β1 (2 ng/mL) for 24 h (a; TGF‐β+), CCI at the indicated doses for 48 h (b) and cycloheximide at 50 μg/mL for the indicated times (c). Intensities of HMGA2 bands normalized to those of GAPDH are shown as ratios relative to the control (N, 0 h) at the bottom in the lower panel.