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. 2012 Oct 30;103(12):2144–2152. doi: 10.1111/cas.12010

Figure 1.

Figure 1

Reaction specificity of cVE199 against rh VEGF‐D. (a) The binding activity of cVE199 to rhVEGF‐D was measured by an ELISA. The various concentrations of cVE199 (black square) and hIgG1 control (white triangle) were reacted to plated hVEGF‐D. Binding was detected by HRP‐labeled anti‐human IgG. (b) The binding specificity of cVE199 to hVEGF‐D was evaluated by immunoprecipitation. (c–e) The inhibitory activity of cVE199 against the binding of (c) hVEGF‐D, (d) mVEGF‐D and (e) hVEGF‐C to VEGFR3 was measured using a cell‐based assay. His‐tagged protein was incubated with VEGFR3 stably expressing CHO cells, in the presence or absence of various concentrations of cVE199 (black square) and hIgG1 control (white triangle). Binding was detected by polyHistidine‐HRP MAb. The absorbance when 1.25 or 0 μg/mL of recombinant protein was added with no antibody is indicated by the (+) or (−) dotted lines, respectively. Points indicate the mean ± SD (n = 3). VEGF, vascular endothelial growth factor.