Figure 1.

Expansion of Tax‐specific CTL from PBMC of patient 8 at different clinical stages. Flow cytometric analyses of the expanded cells are presented. The lymphocyte population was determined by FSC‐H and SSC‐H levels (left panels) and the data are plotted to show CD8 and human leukocyte antigen (HLA)‐A*02:01/Tax tetramer‐positivity (right two panels). Both CD8 and HLA‐A*02:01/Tax tetramer‐positive cells are gated, and their percentages relative to the entire lymphocyte population are indicated in each panel. Patient 8 progressed from chronic to acute stage disease. His Tax‐CTL could be efficiently expanded during the chronic phase (upper panels), but no longer after progression to acute stage (middle panels). Subsequently, he received allogeneic hematopoietic stem cell transplantation, and achieved partial remission. At this time, his Tax‐CTL could be efficiently expanded from PBMC once more (lower panels).