Figure 1.

Antigen fused with heat shock protein (HSP)70 is more efficient in cross‐priming T cells than antigen alone. Recombinant proteins used in this study and their purity as assessed by SDS‐PAGE and Coomassie Brilliant Blue staining (a). Carboxyfluorescein succinimidyl ester (CFSE)‐labeled OT‐I CD8⁺ T cells were adoptively transferred into C57BL/6 mice and simultaneously the indicated dose of ovalbumin (OVA) or OVA‐HSP70 was injected. Three days later, division of OT‐I CD8⁺ T cells in recipient mice spleens was determined by assessing CFSE dilution by flow cytometry (b).