Figure 2.

Heat shock protein (HSP)‐mediated cross‐presentation in vivo is dependent on CD11c⁺ dendritic cells (DC) and transporter associated with antigen processing (TAP). The HSP‐mediated proliferation of transferred carboxyfluorescein succinimidyl ester (CFSE)‐labeled OT‐I CD8⁺ T cells was measured in DC depleted (a), TAP1 KO (b), and CD4⁺ T cell and natural killer (NK) cell depleted (c) recipient mice. Injection of CFSE‐labeled OT‐I CD8⁺ T cells and ovalbumin (OVA)‐HSP70 (10 μg = 100 pmol) and flow cytometry were carried out. In CD11c–diphtheria toxin receptor (DTR) mice, but not WT mice, CD11c⁺ cells were depleted by injection of DT on days −1 and 1 (a). CD4⁺ and NK cells were depleted with injection of GK1.5 (anti‐CD4) and PK136 (anti‐NK1.1) antibodies on day −1 (c).