Figure 3.

CD8⁺ dendritic cells are responsible for heat shock protein (HSP)70‐mediated cross‐priming in vivo. C57BL/6 mice were injected i.v. with 100 μg Alexa Fluor 633‐labeled ovalbumin (OVA)‐HSP70. Three hours or 24 h later, spleen cells of recipient mice were recovered and stained with FITC‐CD11c and PE‐B220, then OVA‐HSP70 uptake was measured by flow cytometry. The numbers indicate the frequency of cells within a particular gate.