Figure 4.

CD8⁺ dendritic cells (DC) showed more efficient ovalbumin–heat shock protein 70 (OVA‐HSP70) uptake and HSP‐mediated cross‐priming than CD8⁻ DC. Uptake of Alexa Fluor 633‐labeled OVA‐HSP70 by CD8⁺ and CD8⁻ DC was detected. Both FITC‐CD8 and PE‐CD11c were used for staining (a). The FACS‐purified CD8⁺ and CD8⁻ DCs were pulsed with 150 μg/mL OVA‐HSP70 for 3 h and fixed. The fixed DC and OT‐I CD8⁺ T cells were co‐cultured in 96‐well round‐bottom plates. Supernatants were collected and the amount of γ‐interferon (IFN‐γ) was measured by ELISA assay (b, left panel). After supernatants were recovered, the co‐cultured OT‐I CD8⁺ T cells were used as effectors in a ⁵¹Cr release assay against E.G7 target cells (b, right panel).