Figure 5.

Heat shock protein (HSP)‐mediated cross‐priming in vivo is independent of Toll‐like receptors (TLR)/MyD88 and CD40. Carboxyfluorescein succinimidyl ester (CFSE)‐labeled OT‐I CD8⁺ T cells were adoptively transferred into C57BL/6 or the indicated KO mice. Simultaneously the mice were injected with 10 μg ovalbumin (OVA)‐HSP70. Three days later, recipient spleen cells were stimulated with OVA _257–264 in the presence of brefeldin A at 37°C for 4 h. After intracellular γ‐interferon (IFN‐γ) staining, IFN‐γ production and proliferation of transferred OT‐I CD8⁺ T cells were determined by flow cytometry.