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. 2012 Oct 30;103(12):2110–2119. doi: 10.1111/cas.12025

Figure 1.

Figure 1

Reduced expression of vacuole membrane protein 1 (Vmp1) in hepatocellular carcinoma (HCC) tissues and HCC cells. (A) Representative results showed expression patterns of Vmp1 mRNA and protein in tissue samples determined using RT‐PCR and western blotting. The independent t test showed that the expression of Vmp1 mRNA and protein in HCC tissues was significantly lower than those in the corresponding paracarcinomatous liver tissue (PCLT) and normal liver (NL) tissues, and even lower in portal vein tumor thrombosis (PVTT) than in HCC. (B) For the quantitative real‐time PCR results, fold inductions were calculated using the formula 2−(ΔΔCT), and the average value of Vmp1 mRNA from PCLT was used to normalize (value set to 1) the corresponding average value of PVTT, HCC and NT. The result of PVTT/PCLT was 0.30, HCC/PCLT was 0.59 and NL/PCLT was 1.08. (C) Spearman's correlation coefficient was used to evaluate the correlation between mRNA and protein expression levels of Vmp1 in HCC. (D) Vmp1 mRNA and protein was examined in HepG2, MHCC97‐L and HCCLM3 HCC cell lines and the CCL13 liver cell line using RT‐PCR and western blotting.