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Construction of vacuole membrane protein 1 (Vmp1) stable overexpressed HCCLM3 cells. The expression of Vmp1 mRNA and protein in HCCLM3 VMP1+ and HCCLM3 vector cells was determined using RT‐PCR and western blot. Lanes 1, 2 and 3 in part (B) represent HCCLM3, HCCLM3vector and HCCLM3VMP1+, respectively.
