Figure 6.
Direct interaction of vacuole membrane protein 1 (Vmp1) and ZO‐1 in hepatocellular carcinoma (HCC) cells. To test the direct interaction between Vmp1 and ZO‐1, co‐immunoprecipitations with HCCLM3VMP1+ cell lysates were performed using a ZO‐1 capture antibody. The cell lysate, flow through and wash before the elution were used as controls. ZO‐1 was visible at 220 kDa in the cell lysate and eluate but not in the flow through and the wash. A band at 46 kDa corresponding to Vmp1 was detected with a Vmp1‐specific antibody in the same lysate and eluate fractions of ZO‐1 precipitates, confirming the direct interaction of these two proteins.