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. 2020 Nov 11;10:19606. doi: 10.1038/s41598-020-76456-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Reduced presence of SV2 in the nAc of 2xTg mice. (a) Immunostaining of coronal slices (30 μm) and high magnification images showing SV2 protein in the nAc from WT and 2xTg mice. (b) Quantification of SV2 fluorescence intensity in the soma of nAc neurons (dashed circles in (a) show regions of interest). Data are mean ± SEM and each data point reflects one soma. Unpaired Student’s t-test (t(90) = 3.243, **p = 0.0017). n = 3 WT and 2xTg mice. Images were analyzed with ‘ImageJ’ 1.8.0_112 (NIH), https://imagej.nih.gov/ij.