Fig. 5. Schematic illustration showing CB₁ forming dynamic peri-periodic hotspots to increase signaling efficiency.

a, b Two-color STED images of CB₁ (magenta) and βII-spectrin (green) in neurons treated with LatB (a) and CytoD (b). N = 3 biological replicates. c Without ligand binding, MPS sets the range for CB₁ distribution. d Upon ligand binding, active CB₁ are recruited to the MPS and become more periodic, making downstream AKT and ERK signaling more effective. e MPS degradation leads to less strong periodic clusters of CB₁ and thus less downstream signaling.