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. 2012 Oct 22;103(12):2102–2109. doi: 10.1111/cas.12022

Figure 3.

Figure 3

Wilms' tumor 1‐associating protein (WTAP) regulates migration of gliomablastoma cells. Boyden chamber assay was used to measure migration of cancer cells. Epidermal growth factor (EGF) (100 ng/mL) or 10% serum was used to induce chemotaxis. (a–c) Boyden chamber assay was performed 2 days after transfection. Knockdown of WTAP significantly inhibited serum‐ or EGF‐induced migration compared to scrambled (SCR) siRNA (100 nm) in U87MG (a,b) and GBM05 cells (c). Overexpression of WTAP significantly increased migration compared to the control vector (d,e). Representative stainings of migrated cells were presented (a,d). Migrated cells were counted and the data are presented as graphs (b,c,e). Data are the means ± SD of three independent experiments in triplicate (*P < 0.01, Student's t‐test).