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. 2020 Nov 11;10:19548. doi: 10.1038/s41598-020-75577-6

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Structure of the elk circovirus Banff/2019 genome. Bottom: Complete circular genome map and coverage plot. On the outside, start and end nucleotide position of ORFs and motifs are indicated in italics. Amino acid sequences (5′–3′) are shown in single letter code beside motif names and positions. ORFs were visualized using SnapGene Viewer v5.0.7 (snapgene.com). Inside the annotated circular genome is a circular plot (generated using GView v1.7³⁸) showing coverage at each nucleotide position based on reference mapping of the liver-derived metagenomic sequencing data to the assembled genome. Inside the coverage plot, an 874 nt PCR product (from nucleotide positon 963 to 49) that was subsequently sequenced in order to confirm a region with low relative coverage, is indicated (coverage data not shown). Top: Expanded view of the origin of replication stem-loop region. Nucleotide position and features of note are labelled. Hexamer repeats are labelled as H1-3 with a reverse orientation repeat on the opposite stem of the stem-loop compared to H1 labelled as H1^R. A single base insertion located in the H2 hexamer repeat sequence that is also found in BatACV11 is indicated in bold and italics.