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. 2012 Oct 10;103(11):2027–2037. doi: 10.1111/j.1349-7006.2012.02412.x

Figure 5.

Figure 5

Regulation of CCL2 by VHL in a hypoxia‐inducible factor (HIF)‐independent manner. (A) Regulation of CCL2 by pVHL and HIF2α variant in the subclones. The expression of CCL2 was evaluated by real‐time PCR with a set of primers different from that of PCR arrays. Columns, mean; bars, SE. (B) Representative pictures of tumors from 786‐O subclones with CCL2 immunostaining. Scale bars = 100 μm. (C) Regulation of CCL2 by pVHL in HIF1α/HIF2α accumulated UMRC2 cells. UMRC2 cells stably transfected with plasmid encoding HA‐tagged wild‐type pVHL (VHL) or empty vector (HA) were used. Top, immunoblot assays with the indicated antibodies. Bottom, CCL2 expression in each cell type. Columns, mean; bars, SE. (D) HIF1α‐independent regulation of CCL2 in UMRC2 cells. UMRC2 cells transfected with lentivirus shRNA to knockdown HIF1α (shHIF1α) or non‐silencing shRNA (Scr) were examined. Top left, immunoblot assays with the indicated antibodies. Top right, CCL2 expression in each cell type. Bottom, BNIP3 and HK2 expression in each cell type. Columns, mean; bars, SE.