Table 2.
mRNA changes between pRC3 versus WT8/HIF2aP531A
| Unigene | Symbol | Description | Fold change (pRC3/WT8) A | Fold change (HIF2aP531A/mock) B | Ratio A/B |
|---|---|---|---|---|---|
| Genes upregulated | |||||
| Hs.303649 | CCL2 | Chemokine (C‐C motif) ligand 2 | 88.91 | 0.60 | 149.19 |
| Hs.252820 | PGF | Placental growth factor | 30.75 | 2.82 | 10.91 |
| Hs.513617 | MMP2 | Matrix metallopeptidase 2 | 15.91 | 8.67 | 1.84 |
| Hs.297413 | MMP9 | Matrix metallopeptidase 9 | 8.05 | 3.19 | 2.52 |
| Hs.471200 | NRP2 | Neuropilin 2 | 7.60 | 1.12 | 6.76 |
| Hs.149239 | EFNB2 | Ephrin‐B2 | 4.00 | 1.26 | 3.17 |
| Hs.164226 | THBS1 | Thrombospondin 1 | 3.57 | 0.65 | 5.49 |
| Hs.371147 | THBS2 | Thrombospondin 2 | 3.42 | 0.41 | 8.32 |
| Hs.435215 | VEGFC | Vascular endothelial growth factor C | 3.38 | 2.35 | 1.44 |
| Hs.437008 | EPHB4 | EPH receptor B4 | 2.71 | 1.32 | 2.06 |
| Hs.133379 | TGFB2 | Transforming growth factor, beta 2 | 2.69 | 0.55 | 4.89 |
| Hs.76884 | ID3 | Inhibitor of DNA binding 3 | 2.64 | 1.23 | 2.14 |
| Hs.517356 | COL18A1 | Collagen, type XVIII, alpha 1 | 2.24 | 0.68 | 3.28 |
| Hs.73793 | VEGFA | Vascular endothelial growth factor A | 2.12 | 2.05 | 1.03 |
The RT2 Profiler human angiogenesis PCR Arrays (SA Biosciences) were carried out according to the manufacturer's protocols. Expression levels of genes involved in angiogenesis were compared between WT8 and pRC3, WT8/HIF2a P531A, and WT8/mock cells. Five housekeeping genes were used as controls for each gene expression calculation, and the extent of change in expression of each gene was calculated using the DCt method. Only genes whose expression was upregulated or downregulated at least 2‐fold are shown in Table 2. When DCt was over 12, and therefore the expression was thought to be extremely low, the gene was omitted from the analysis.