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. 2012 Feb 3;103(4):813–820. doi: 10.1111/j.1349-7006.2011.02199.x

Figure 3.

Figure 3

Effect of zinc finger E‐box binding homeobox 1 (ZEB1) on the vasculogenic mimicry (VM) formation of three colorectal carcinoma (CRC) cell lines in Matrigel. (A) HCT116 cells can form typical tube‐like structures in the 3‐D culture (red arrow), whereas HT‐29 and SW480 cannot. The typical tube‐like structures formed by human umbilical vein endothelial cells (HUVEC) were regarded as positive control. After ZEB1 siRNA treatment, VM formation was significantly reduced in HCT116 cells. (B) Quantitative analysis of the mean number of tube‐like structures formed by HUVEC, control HCT116 and siZEB1 HCT116 cells in 3‐D culture. (C) Western blot analysis showed that the ZEB1 knockdown in HCT116 cells results in reduced VE‐cadherin and Flk‐1 expression. No significant change in Flt‐1 expression was observed.