Modification of Dysadherin in breast cancer cell lines. (A,B) qRT‐PCR and immunoblot analysis for dysadherin in breast cancer cell lines. (C,D) qRT‐PCR and immunoblot analysis for dysadherin in control (none, non‐transfected cells), mock (empty vector), Dys (Dysadherin cDNA), shCon (control shRNA) or shDys‐ (Dysadherin shRNA) transfected cells. Dysadherin mRNA were normalized with peptidylprolyl isomerase A mRNA in each sample. β‐actin was used as a loading control in the immunoblot analysis. Data are the mean ± SD (n = 3). *P < 0.01. NS, not significant.