Figure 5.

Functions of Bach1, MDM2, and p19^ARF in the regulation of p53. (a) A proposed model of p19^ARF–p53 pathways through MDM2 (canonical pathway) and Bach1 (alternative pathway). (b) Proliferation of pre‐senescent wild‐type or Bach1‐deficient mouse embryonic fibroblasts expressing Stealth RNAi duplexes of control (Ctrl RNAi) or MDM2 or p19 ^ARF is compared at day 5 as values relative to day 1. The experiments were carried out independently three times in triplicate and are expressed as means ± SD. (c) p19^ARF and MDM2 mRNA levels 2 days after RNAi were determined by quantitative RT‐PCR as relative mean values ± SD compared to day 1 control RNAi.