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. 2020 Oct 29;7:561866. doi: 10.3389/fmed.2020.561866

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Copyright © 2020 Hachim, Elemam, Ramakrishnan, Salameh, Olivenstein, Hachim, Venkatachalam, Mahboub, Al Heialy, Halwani, Hamid and Hamoudi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AREG mRNA expression as measured by RT qPCR in PBMCs and saliva of cohort of asthmatic vs. healthy control. (A) AREG mRNA level in PBMCs of the locally recruited cohort (cohort 1), healthy controls (n = 13), non-severe asthmatics (n = 20), and severe asthmatic (n = 18). (B) AREG mRNA gene expression in PBMC of a locally recruited cohort divided according to the ACT score into two groups: a well-controlled group (ACT > 20) and those with poor control (ACT < 20). (C) AREG mRNA gene expression in the saliva of independent validation locally recruited cohort (cohort 2), which includes non-severe asthmatics (n = 21) and severe asthmatic who are not on biological treatment (n = 17).