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. 2020 Sep 24;219(10):e201912074. doi: 10.1083/jcb.201912074

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© 2020 Wozniak et al.

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Figure 2. — RILP cleavage status regulates exosome cargo loading and specificity. (A) Inflammasome activation using LPS/ATP leads to the secretion of proinflammatory miRNAs within the exosome (black bars). Note that some miRs are unchanged by treatment with LPS/ATP (white bars), while at least one decreases (gray bars). *, P ≤ 0.05 compared with untreated for n = 4–8. (B) Exosomes derived from cRILP-expressing cells are enriched in miR-155, while miR19b remains unchanged. *, P ≤ 0.05 compared with empty vector for n = 4. (C) qPCR analysis of miRNAs in both exosome and cell-associated fractions showing that ncRILP blocks the secretion of a miR-155–containing exosome after inflammasome activation by LPS and ATP. miR19b is unaffected by the expression of ncRILP. (D) Sequence alignments of miRs that are up-regulated by cRILP. (E) A common motif is present in exosome miRNAs that are up-regulated by cRILP. (F) miR-195, an miRNA that does not contain the AAUGC motif, is enriched in exosomes isolated from LPS/ATP-treated cells. The expression of ncRILP further enriches this miR within the exosome. Data are shown as mean ± SD; *, P ≤ 0.05 for n = 4–8.