Figure S3.

Effects of nuclear localization of the CB construct on its tension and of wounding and HGF on the CH mutant construct. (A) FRET index difference between the front and back of a nucleus within leader cells (n = 22); one replicate. Mean ± SEM. Two-tailed Kruskal-Wallis test. (B) FRET index map of a wounded MDCK monolayer expressing the CH construct. Compare with Fig. 3 A. Scale bar = 10 µm. (C) Direct fluorescence and FRET index maps of the CH construct in MDCK cells after 5 h with or without HGF. Compare with Fig. 3 E. Scale bar = 10 µm.