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. 2020 Aug 13;219(10):e201908036. doi: 10.1083/jcb.201908036

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© 2020 Déjardin et al.

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Figure S5. — FRET calibration and tension dependence on z. (A) FRET index of 5aa and TRAF standards expressed in MDCK cells (n = 4); one replicate. (B) Left: Sketch of a cell growing on the side of a PDMS channel for FRET analysis along z. Direct fluorescence and FRET index of an MDCK cell stably expressing the CB construct from the region in the dotted box above. Right: Normalized FRET index from the white dotted boxes on the left (n = 4); one replicate. Scale bar = 5 µm. Mean ± SEM. Two-tailed Kruskal-Wallis test. *, P < 0.05.