Video 9.
ISMic imaging of WT neutrophils introduced into Alox5−/− mouse with inflamed footpad ∼2 h, where EVs contain CellTracker Green CMFDA (green) and Itgb2 (M18/2 clone; red). Videos are maximum-intensity projections of an image stack and represent ∼11 min (min:s:ms) of acquisition. The vessels were labeled with anti-CD31 (cyan). Scale bar = 3 µm. The EVs roll along and occasionally tether to the inflamed endothelial surface facing the vascular lumen in the vicinity of extravasating neutrophils.