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. Author manuscript; available in PMC: 2020 Nov 12.
Published in final edited form as: Mol Cell. 2013 Aug 22;51(4):480–492. doi: 10.1016/j.molcel.2013.08.009

Figure 3.

Figure 3

Differential Expression of H1 and H2A during S-phase. (A) Co-staining of H1 or H2A mRNA through FISH (Red) with EdU incorporation (green), marking S-phase. (B) Schematic of the double pulse chase experiment for S-phase timing. (C) Co-staining of BrdU (green) with EdU (red) when using a 90 minutes chase between BrdU and EdU incubations. (D) Upper panel, Association of EdU staining patterns with BrdU staining as a function of chase time. Each colored graph (A to F) represents one of 6 distinct EdU staining patterns. Lower panel, Repartition of EdU staining patterns across S-phase. Each colored bar represents one independently detectable EdU staining pattern (N=80-166). Lower Panel, The length of the bar on the arrow represents approximately when an EdU pattern is occurring the most during S-phase. (E) Fraction of cells that are stained by Edu and BrdU both in function of chase time. (F) Fraction of cells with nascent H2A mRNA for cells within each different EdU staining pattern (N=68-103). Each colored bar corresponds to a EdU staining pattern with the same color code from panel (D).