TBP depends on TFIIA but not HLB factors, TFIIB or TFIID for binding to the histone locus. (A) Representative co-staining of TBP (green) with Lsm11 (red) in S2 cells after dsRNA treatment. From first to last row, cells after dsRNA treatment against control, Lsm11, Mxc, TFIIA-L, TFIIB, TAF4. (B) Fraction of TBP dots at His-C showing co-localization with a Lsm11 signal in cells depleted of Lsm11 or Mxc. Average of 2-3 RNAi experiments with standard deviations (N=96-169). (C) Fraction of Lsm11 dots at His-C showing co-localization with a TBP signal in cells depleted of TFIIA-L. Average of 2-3 RNAi experiments with standard deviations.