Figure 2.

Monarda essential oil-induced increases in [Ca²⁺]_i are blocked by HC-030031 in HEK rTRPA1 cells. (A) and (B) are representative traces showing the effects of pretreatment with the selective TRPA1 blocker HC-030031 (20 µM) on the transient increase in [Ca²⁺]_i in HEK-rTRPA1 cells. Summarized data are shown in (C) and (D), respectively. The data are expressed as changes in the [Ca²⁺]_i influx from baseline to peak [Ca²⁺]_i and plotted as the mean ± SEM. * p < 0.0001 compared to [Ca²⁺]_i elicited after treatment with essential oils. Statistical analysis was performed using an unpaired t-test; n = 90 cells from 3 independent experiments.