Figure 4.

TRP agonist- and Monarda essential oil-induced increases in [Ca²⁺]_i are absent in parental HEK cells. Representative trace (A) showing the negligible effect in baseline [Ca²⁺]_i in parental HEK 293 cells treated consecutively with 100 µM of allyl isothiocyanate (AITC) (#1), 100 nM of capsaicin (#2), 10 µM of GSK1016790A (#3), 20 µg/mL of MEO_Fl (#5), and 20 µg/mL of MEO_Lv (#6). Receptor-effector coupling was confirmed by treating the cells with 25 µM of ATP (#6). Summarized data comparing the effects of MEO_Fl and MEO_Lv on [Ca²⁺]_i in parental HEK 293 and HEK rTRPA1 cells (B). The data are expressed as change in the [Ca²⁺]_i influx from baseline to peak [Ca²⁺]_i and plotted as the mean ± SEM. * p < 0.0001 compared to agonist-induced [Ca²⁺]_i influx. Statistical analysis was performed using two-way analysis of variance; n = 90 cells from 3 independent experiments.