Table 4.
Compound | Composition | HEK Cells | ||
---|---|---|---|---|
MEOFl | MEOLv | rTRPA1 | NT | |
% | [Ca2+]i Change | |||
α-Pinene | 0.7 | 0.4 | 0.054 ± 0.014 | 0.013 ± 0.002 |
Camphene | 0.1 | 0.1 | 0.014 ± 0.004 | 0.009 ± 0.001 |
β-Pinene | 0.2 | 0.1 | 0.012 ± 0.002 | 0.011 ± 0.001 |
Sabinene | 0.3 | 0.1 | 0.029 ± 0.005 | 0.034 ± 0.004 |
β-Myrcene | 1.4 | 0.8 | 0.111 ± 0.003 | 0.005 ± 0.001 |
α-Terpinene | 3.8 | 1.7 | 0.007 ± 0.001 | 0.006 ± 0.001 |
(-) Limonene | 0.9 | 0.5 | 0.008 ± 0.002 | 0.007 ± 0.001 |
(+) Limonene | - | - | 0.011 ± 0.002 | 0.009 ± 0.002 |
γ-Terpinene | 6.8 | 2.5 | 0.034 ± 0.005 | 0.014 ± 0.001 |
p-Cymene | 25.6 | 13.1 | 0.071 ± 0.011 | 0.027 ± 0.001 |
Terpinolene | 0.1 | - | 0.034 ± 0.007 | 0.022 ± 0.003 |
Linalool | 0.1 | 0.1 | 0.037 ± 0.007 | 0.002 ± 0.0003 |
Terpinen-4-ol | 1.1 | - | 0.046 ± 0.007 | 0.013 ± 0.002 |
α-Terpineol | 0.2 | 0.1 | 0.010 ± 0.002 | 0.004 ± 0.001 |
Thymol | 3.1 | 3.3 | 0.849 ± 0.013 | 0.026 ± 0.002 |
Carvacrol | 45.7 | 71.5 | 0.340 ± 0.012 | 0.061 ± 0.018 |
Values of [Ca2+]i changes are presented as the mean ± S.E.M. of 3 independent experiments, as described in Materials and Methods. The cells were treated with 100 µM of monoterpene, and [Ca2+]i was monitored, as described. NT, nontransfected HEK cells.