Figure 9.

Binding of biotin-labeled recombinant fungal enolase to microplate-immobilized VTR, FN and HPG. Microplate wells coated with 3 pmoles of VTR, FN or HPG were filled with 50 µL of biotin-labeled recombinant enolases in increasing concentrations. The absorbance intensities were measured after the washing of unbound proteins. The results obtained during the control experiment (non-human protein surface blocked with 3% BSA solution) were subtracted from the total binding measurements. The bars represent the mean values ± standard deviation (3 determinations). Statistical significance was determined using one-way ANOVA; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.