Table 1.

Kinetic and thermodynamic parameters for the interactions of C. albicans and C. tropicalis enolases with VTR, FN or HPG, determined by SPR measurements. The SPR-determined k_a, k_d and K_D values were obtained for enolase as a ligand and for human proteins as an analyte. All parameters are presented with the standard error.

Human Protein	Enolase Origin	KD [M]	ka [1/Ms]	kd [1/s]
VTR	C. albicans, cell wall	3.11 × 10−8 ± 4.58 × 10−10	1.34 × 105 ± 2.52 × 103	4.17 × 10−3 ± 7.63 × 10−5
VTR	C. albicans, cytosol	5.53 × 10−8 ± 2.43 × 10−9	8.91 × 104 ± 5.06 × 103	4.93 × 10−3 ± 9.12 × 10−5
VTR	C. tropicalis, cytosol	6.83 × 10−7 ± 4.39 × 10−8	1.34 × 104 ± 1.11 × 103	9.15 × 10−3 ± 5.96 × 10−4
FN	C. albicans, cell wall	3.68 × 10−8 ± 3.26 × 10−9	6.03 × 104 ± 1.45 × 103	2.22 × 10−3 ± 6.74 × 10−5
FN	C. albicans, cytosol	4.28 × 10−8 ± 3.74 × 10−9	6.47 × 105 ± 9.84 × 103	2.77 × 10−2 ± 3.58 × 10−3
FN	C. tropicalis, cytosol	5.18 × 10−8 ± 8.54 × 10−10	5.48 × 104 ± 8.41 × 102	2.84 × 10−3 ± 6.04 × 10−5
HPG	C. albicans, cell wall	1.20 × 10−7 ± 4.25 × 10−6	5.22 × 105 ± 4.84 × 104	6.26 × 10−2 ± 2.26 × 10−3
HPG	C. albicans, cytosol	2.57 × 10−7 ± 4.17 × 10−6	2.70 × 105 ± 1.92 × 104	6.93 × 10−2 ± 1.30 × 10−3
HPG	C. tropicalis, cytosol	2.53 × 10−7 ± 2.06 × 10−6	5.77 × 104 ± 6.84 × 103	1.46 × 10−2 ± 2.18 × 10−1

VTR: vitronectin; FN: fibronectin; HPG: human plasminogen.