Table 2:

Summary of the H1.5 levels and their effects

Cases of H1.5 down-regulation	Cases of H1.5 up-regulation
NT2 differentiation into neural cells [10] Retinoic acid-induced differentiation of hESCs (relative contribution to H1 content measured by mRNA) [10] General finding in cancer cell lines [20] Granulosa cell tumour—no H1.5 compared to healthy ovarian tissue [37] H1.5T10ph in gliomas [21]	De-differentiation of keratinocytes to iPS [10] ↑ Growth/metastasis of prostate/uterine smooth muscle cancer [31, 32, 36] Differentiation of hESCs to lung fibroblasts (mRNA) [16]
H1.5 KO/KD experiments	H1.5 overexpression experiments
shRNA in fibroblasts →↓ chromatin compaction → dysregulation of 10% of genes +↓ cell growth [20] siRNA in glioblastoma cells → reactivation of apoptotic pathways [39] siRNA → ↓ inclusion of alternatively spliced exons [16]	Viral vector in oocytes → no increase in NRL [15] H1.5T10ph mimic in glioma cells → reversal of mutant Ras-mediated changes in gene expression and phenotype (↓ colony formation and migration) [21]