Figure 5.

Analysis of synaptic and extrasynaptic AChR expression, and expression of key NMJ genes in M-LAP1^−/− mice and littermate controls. (A) Synaptic and extrasynaptic AChR expression levels in hemidiaphragm preparations were measured using ¹²⁵I-α-BuTx. Synaptic levels of AChR are unchanged in the M-LAP1^−/− mice, but AChR expression is increased in extrasynaptic regions. Three mice age 12 weeks per group; *P < 0.05. (B) RNA-seq analysis of differential expression of key NMJ genes. RNA extracted from EDL muscles from 12 week old M-LAP1^−/− mice and littermate controls were compared, three mice per group. (C) qPCR analysis of expression of genes encoding the AChR subunits, MuSK, Dok7 and rapsyn. Expression of each gene was compared with expression of Hprt1, which was used as a housekeeping gene control. The fold change between the control and M-LAP1^−/− mice was then calculated.