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. 2020 Aug 4;6(10):mgen000417. doi: 10.1099/mgen.0.000417

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© 2020 The Authors

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Fig. 4. — Input DNA mass correction allows accurate quantification of the 16S copy number in degraded metagenomic DNAs. For five DIN1 faecal DNAs (samples F9–F13), a mass correction of the DNA template in the ddPCR reaction, based on the 55 % underestimation percentage (DIN1 input corrected, DIN1-IC), allowed us to obtain the total number of 16S copies (a) and the number of A. muciniphila 16S copies (b) corresponding to those measured in the original DIN6 DNA samples. *, P<0.05, **, P<0.01, ***, P<0,001 vs DIN1.