Fig. 2.

Determination of the acceptor specificity of the purified enzymes U1 (a), U3 (b) and U7 (c). The reactions were performed using 10 mM of αMan1P (glycosyl donor) and 10 mM of acceptor. The release of inorganic phosphate was followed using the molybdenum blue activity assay. Reactions were performed in triplicate. When no reverse phosphorolysis activity was detected (similar inorganic phosphate release rate to that in the presence of αMan1P as sole substrate), the data do not appear in this figure.