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. Author manuscript; available in PMC: 2020 Nov 12.
Published in final edited form as: Cell Rep. 2020 Oct 27;33(4):108270. doi: 10.1016/j.celrep.2020.108270

Figure 2. RYGB Induces an Increase in Markers of Gut’s Sympathetic Nerve Activity.

Figure 2.

(A) TH expression—assessed by immunofluorescence (IF)—in the Roux limb (or jejunum) of DIO C57BL/6 mice after RYGB compared with their sham counterparts 6 weeks after surgery. Sham n = 5, RYGB n = 5.

(B) TH and CGRP expression—assessed by western blot—within the Roux limb (jejunum) as well as TH expression within vWAT of RYGB- and sham-operated DIO mice 6 weeks after surgery. β-Actin serviced as a loading control.

(C) TH expression—assessed by IF—in the Roux limb (or jejunum) of DIO rhesus macaque monkeys after RYGB compared with their sham counterparts 12 weeks after surgery. Sham n = 4, RYGB n = 4.

(D) ADBR3 and TH expression in Roux (or proximal jejunum)—assessed by IHC—of obese human subjects at time of surgery (before RYGB) and 1–5 years afterward (after RYGB). n = 4–5. Scale bar, 100 μm (A, C, and D).