| Antibodies |
|
|
| Goat polyclonal anti-UCP1 |
Santa Cruz |
Cat# sc-6528, RRID: AB_2304265 |
| Rabbit polyclonal anti-Tyrosine Hydroxylase |
PhosphoSolution |
Cat# 2025-THRAB, RRID: AB_2492276 |
| Rabbit polyclonal anti-CGRP |
Sigma |
Cat# C8198, RRID: AB_259091 |
| Mouse monoclonal anti-β-actin |
Proteintech |
Cat# 60008–1-Ig, RRID: AB_2289225 |
| Mouse monoclonal anti-GAPDH |
Santa Cruz Biotechnology |
Cat# sc-32233, RRID: AB_627679 |
| Rabbit polyclonal anti-CB1 |
Abcam |
Cat# ab23703, RRID: AB_447623 |
| Alexa 488 goat anti-rabbit |
Thermo Fisher Scientific |
Cat# A32731, RRID: AB_2633280 |
| Alexa 568 donkey anti-goat |
Thermo Fisher Scientific |
Cat# A-11057, RRID: AB_2534104 |
| Biological Samples |
|
|
| Human Jejunum samples (details in Table S1) |
Paris Diderot University hospital, Paris, France |
N/A |
| Human gastric samples (details in Table S2) |
University of Iowa Hospitals and Clinics, Iowa City, IA |
N/A |
| Chemicals, Peptides, and Recombinant Proteins |
|
|
| Rimonabant (SR 141716A) |
Tocris Bioscience |
Cat# 0923, CAS: 158681–13-1 |
| Rimonabant (SR 141716A) |
Sigma |
Cat# 0800–25mg |
| Arachidonylethanolamide Oil (AEA) |
Sigma |
Cat# A0580–25mg, CAS:94421–68-8 |
| D-Glucose |
RPI |
CAS: 50–99-7 |
| insulin (Humulin R) |
Eli Lily |
HI-210, NDC 0002–8215-01 |
| silicone gel |
World Precision Instruments |
KWIK-SIL |
| Critical Commercial Assays |
|
|
| Direct-zol RNA MiniPrep Plus kit |
Zymo Research |
Cat# R2072 |
| cDNA High Capacity Reverse Transcription Kit |
Applied Biosystems |
Cat# 4368814 |
| iQ™ SYBR® Green Supermix |
Bio-Rad |
Cat# 170–8882 |
| Chemiluminescence (ECL) detection kit |
GE Healthcare |
Cat# RPN2232 |
| Mouse metabolic kit |
MSD |
Cat# K15124C-1 |
| U-PLEX Mouse PYY (total) Assay |
MSD |
Cat# K1526BK |
| Total bile acids kit |
Crystal Chem |
80470 |
| Experimental Models: Organisms/Strains |
|
|
| Mouse: Male DIO C57BL/6J |
Jackson Laboratory |
JAX: 380050 |
| Mouse: Male C57BL/6J |
Jackson Laboratory |
JAX: 000664 |
| Primates: Male Rhesus Macaques |
Oregon National Primate Research Center, Beaverton, OR |
N/A |
| Oligonucleotides |
|
|
| β3-ADR Forward: GCC TTC CGTCGT GGT CTT CTG TG |
Invitrogen |
N/A |
| β3-ADR Reverse: GCC ATC AAA CCT GTT GAG C |
Invitrogen |
N/A |
| UCP1 Forward: GGA TGG TGA ACC CGA CAA C |
Invitrogen |
N/A |
| UCP1 Reverse: CTT GGA TCT GAA GGC GGA C |
Invitrogen |
N/A |
| PRDM16 Forward: GTA GCT GCT TCT GGG CTC A |
IDT |
N/A |
| PRDM16 Reverse: CGT CAC CGT CAC TTT TGG CT |
IDT |
N/A |
| CB1 Forward: GGG CAA ATT TCC TTG TAG CAG |
IDT |
N/A |
| CB1 Reverse: CTC AGT CTT TGA TTA GGC CAG G |
IDT |
N/A |
| rps18 Forward: CTG CCA TTA AGG GCG TGG |
Invitrogen |
N/A |
| rps18 reverse: TGA TCA CTC GCT CCA CCT CA |
Invitrogen |
N/A |
| Software and Algorithms |
|
|
| ImageJ |
NIH |
https://imagej.nih.gov/ij/download.html |
| Graphpad prism 7.0 |
GraphPad Software |
https://www.graphpad.com |
| Research IR software |
FLIR |
version 3.4.13039.1003 |
| SPSS |
IBM |
https://www.ibm.com/products/spss-statistics |
| Other |
|
|
| Thermal Imager |
FLIR Systems |
A655sc |
| bipolar platinum-iridium wire |
A-M Systems |
36-gauge |
| High impedance probe |
Grass Instruments |
HIP511 |
| AC preamplifier |
Grass Instruments |
P5 |
| Voltage integrator |
University of Iowa Bioengineering |
RVI: Model B600c |
| CLAMs system |
Metabolic Phenotyping Core- University of Iowa |
N/A |
| Combination of Direct and Indirect Calorimetry System |
N/A |
Burnett and Grobe, 2013; Walsberg and Hoffman, 2005
|
| Bomb calorimetry |
University of Arkansas Center of Excellence for Poultry Science, Fayetteville, AR |
N/A |
| Nerve data acquisition system |
MacLab |
Model 8 s |
| Glucometer |
Bayer HealthCare LLC |
Contour |
| Catecholamine concentration (epinephrine (EPI) and norepinephrine (NE)) measurement |
Vanderbilt University. |
Hormone Assay and Analytical Resource Core |
