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. 2020 Oct 23;21(21):7866. doi: 10.3390/ijms21217866

Figure 1.

Figure 1

Generation of Purkinje cell-specific Diacylglycerol kinase γ (DGKγ) knockout (KO) (tm1d) mice and PCR genotyping: tm1c and tm1d alleles encodes DGKγ (a) and Cre recombinase knock-in L7 (b). Cre, Cre recombinase; FRT, flippase recognition target; loxP, Cre recombinase recognition sequence; PGKneopA, PGK-neomycin cassette. PCR genotyping of DGKγgene (c) and Cre recombinase (d) was determined using tail-derived genome: bands at 975, 482, and 292 bp were expected for the DGKγ, Cre, and L7 alleles, respectively. Red arrows show the primer sites for PCR genotyping. (e) Cerebral and cerebellar lysates from tm1c and tm1d mice were subjected to Western blotting and probed with an anti-DGKγ antibody. Quantification of the expression levels of DGKγ was performed by ImageJ. The expression level of DGKγ was normalized to the expression level of the loading control (Glyceraldehyde 3-phosphate dehydrogenase: GAPDH). (n = 3); * p <  0.05, followed by Student’s t-test. Data are expressed as mean ± SEM.