Table 2. BLAST results using full length ITS sequences compared to ITS sequences trimmed to the GenBank reference panel alignment (618 bp) used in phylogenetic analysis.
| Full Length | Trimmed to Alignment Length | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Sample Name | Presumed Species1 | Genbank Accession | Sequence Length (bp) | Top BLAST Hit2 | GenBank Query Coverage | GenBank Percent Similarity | Top BLAST Hit2 | GenBank Query Coverage | GenBank Percent Similarity |
| Powder #1 | G. lucidum | MT994154 | 824 | G. lucidum (MF476200.1) | 100% | 100% | G. lingzhi (MH160076.1) | 100% | 100% |
| Powder #2 | G. lucidum | MT994155 | 739 | G. lucidum (MF476201.1) | 99% | 99% | G. lingzhi (MH160076.1) | 99% | 99% |
| Powder #3 | G. lucidum | MT994156 | 868 | G. lucidum (MF476200.1) | 100% | 100% | G. lingzhi (MH160076.1) | 100% | 100% |
| Powder #4 | G. lucidum | MT994157 | 868 | G. lucidum (MF476200.1) | 100% | 100% | G. lingzhi (MH160076.1) | 100% | 100% |
| Powder #5 | G. lucidum | MT994158 | 865 | G. lucidum (MF476200.1) | 100% | 100% | G. lingzhi (MH160076.1) | 100% | 100% |
| Powder #6 | G. lucidum | MT994159 | 844 | G. lucidum (MF476200.1) | 100% | 99% | G. lingzhi (MH160076.1) | 100% | 100% |
| Fresh #1 | G. brownii | MT994160 | 848 | G. australe (MK968731.1) | 100% | 97% | G. brownii (MG279159.1) | 100% | 100% |
| Fresh #2 | Fomitopsis pinicola | MT994161 | 780 | F. pinicola (EF530947.1) | 100% | 99% | F. pinicola (EF530947.1) | 100% | 99% |
| Fresh #3 | G. lucidum | MT994162 | 868 | G. lucidum (MF476200.1) | 100% | 99% | G. lingzhi (MH160076.1) | 100% | 100% |
1Presumed species is based on product label for store-bought samples and morphological identification [49] for wild-collected samples.
2All top BLAST hits had an E-value of 0.0.