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. 2020 Nov 2;16(11):e1009104. doi: 10.1371/journal.pgen.1009104

Fig 5. The R402H mutation alters the microtubule proteome.

Fig 5

(A) Graphical depiction of the protocol employed to isolate microtubules and microtubule-associated proteins (MAPs) from the forebrain of E18.5 R402H/R402H; Emx1-cre mutants and R402H/R402H littermate controls (n = 4). (B) Volcano plot showing the results of our mass spectrometry analysis. In total we identified 5283 proteins, of which 1330 were significantly altered in R402H/R402H; Emx1-cre mutants (P<0.01) (shown in grey). These were narrowed to 286 proteins upon application of a false discovery rate of 5% (shown in blue). (C) Pyramid showing how we filtered our mass spectrometry dataset, resulting in the identification of seven known MAPs highlighted in orange in (B). (D) Gene ontology analysis performed with g:Profiler on the 286 proteins revealing enrichment for GO terms associated with cellular organization and nervous system development (highlighted in blue).