Fig 3. Images of blood smears of parasite cultures obtained 66 hours post invasion.

Top left: Untreated (DMSO control) showing infected RBCs have progressed to the ring stage of their second life cycle after successful egress at the end of their first life cycle). Bottom left: Positive control of parasites treated with the well-known Syk inhibitor II showing stalled egress at the end of the first life cycle. Panels 1, 2, 3 and 4: Blood smears displaying the stages at which the de-identified drug terminated parasite maturation. Inhibitors 47F and 23D halted parasite maturation at ring stage, while 32C and 32D appear to halt the parasite at the trophozoite stage. Inhibitors 23B3 and 32A3 interrupted parasite development at the schizont stage. Syk phenotype inhibitors; 32E, 47H, 47A3 and 26D appear to stop the parasite from egressing at the segmenter stage as evidenced by the separated merozoites within infected cells.