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. 2020 Nov 12;15(11):e0241592. doi: 10.1371/journal.pone.0241592

Fig 4. After primary infection, all cells contain viral RNA.

Fig 4

(A) HFFs were infected with CHIKV-GFP at MOI 1 for 24 h, then harvested and separated into two samples for analysis by flow cytometry and single-cell RNA-sequencing using 10x genomics Single Cell 3’. (B) Flow cytometry analysis of the infected cells. (C) T-SNE analysis of the RNA-sequencing data. (D) Distribution of the number of CHIKV unique molecular identifiers (UMI) per cell.