Fig 5. De novo host cell transcription is dispensable for the establishment of CHIKV SIE.

BHK cells, pre-treated with ActD or DMSO, were infected with CHIKV-GFP for 8 h at the indicated MOI, then with CHIKV-mCherry at MOI 1 for 8 h. Quantification of Ifnb1 and Cxcl10 RNA (A), infected cells (B) and representative flow cytometry plot (C) are shown. Bars indicate mean ± SD of biological triplicates, and data are representative of two independent experiments. NS, not significant; ***p < 0.001 (one-way analysis of variance followed by Dunnett’s post-test).