Table 1. Real-time qPCR primers.

Gene	Forward primer (5’- 3’)	Tm	Reverse primer (5–3’)	Tm
β-ACTIN	TTG CCG ACA GGA TGC AGA AGG A	61	AGG TGG ACA GCG AGG CCA GGA T	65
c-MYC	AAA CAC AAA CTT GAA CAG CTA C	51.8	ATT TGA GGC AGT TTA CAT TAT GG	51.4
OCT4A	CTC CTG GAG GGC CAG GAA TC	59.8	CCA CAT CGG CCT GTG TAT AT	54.4
KLF4	ACC AGG CAC TAC CGT AAA CAC A	58.5	GGT CCG ACC TGG AAA ATG CT	57.7
SOX2	CCC AGC AGA CTTC ACA TGT	55	CCT CCC ATT TCC CTC GTTTT	55.1
NANOG	TGA ACC TCA GCT ACA AAC AG	52.3	TGG TGG TAG GAA GAG TAA AG	51
TET1	CAG AAC CTA AAC CAC CCG TG	64.2	TGC TTC GTA GCG CCA TTG TAA	67.5
CBS	TCA AGA GCA ACG ATG AGG AG	54.4	ATG TAG TTC CGC ACT GAG TC	54.7
CSE	AGA AGG TGA TTG ACA TTG AAG G	61.9	CAA TAG GAG ATG GAA CTG CTC	60.3
MPST	CGC CGT GTC ACT GCT TGA T	58.3	CAC CTG GAA GCG CCG GGA TT	62.9
DNMT1	CCT AGC CCC AGG ATT ACA AGG	61.4	ACT CATC CGA TTT GGC TCT TTC	60.4
RUNX2	ATG CGC CCT AAA TCA CTG AG	55.1	GTC TTC ACA AAT CCT CCC CA	54.5
OCN	GTG CAG AGT CCA GCA AAG GT	57.8	AGC AGA GCG ACA CCC TAG AC	58.8
ALP	AAC GTG GCC AAG AAC ATC ATC A	57.2	TGT CCA TCT CCA GCC GTG TC	59.4